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This Rac1 G-LISA Activation Assay Kit is a colorimetric based assay and shares the many advantages that all G-LISA activation assay have. This assay is specific for Rac1.The kit contains a Rac-GTP-binding protein linked to the wells of a 96 well plate. Active GTP-bound Rac1 is captured by this protein while inactive GDP-bound Rac1 is removed following washes. The active Rac1 bound to the wells is detected with a Rac1 specific antibody. The degree of Rac1 activation is determined by comparing readings from activated cell lysates versus non-activated cell lysates.
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