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===under info was translated buy computer===
Rap Activation Kit provides a rapid, cost-effective and reliable tool for the detection and semi-quantitative analysis of the cellular activation state of Rap GTPases. The kit exploits the selective interaction of the Ras-interaction domain (RA) of the Ras/Rap-effector RalGDS with the active, Rap-GTP conformation. Recombinant, GST-tagged RalGDS-RA is added to cell extracts to pull out Rap-GTP, which is consequently detected by Western blotting. The kit detects active, GTP-bound Rap1A, Rap1B, Rap2A and Rap2B. However, the antibody included in the kit allows detection of Rap1 isoforms only. The kit also includes recombinant Rap1A protein that, once preloaded with GDP or GMPpNHp (all included in the kit), can be used to "spike" the cell extracts thus serving as an internal control of signal specificity.
https://www.creativebiomart.net/rap-activation-kit-463693.htm
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