===under info was translated buy computer===
Ras Activation Kit provides a rapid, cost-effective and reliable tool for the detection and semi-quantitative analysis of the cellular activation state of the three prototypical Ras proteins K-Ras, N-Ras and H-Ras (collectively referred to as Ras). Active Ras, i. e. Ras complexed to GTP, is purified from cell extracts via a one-step batch affinity purification step employing GST-RBD, an affinity probe that selectively interacts with the active, GTP-bound Ras conformation in loss of the inactive Ras-GDP complex. The kit further includes recombinant H-Ras. K-Ras and N-Ras proteins serve dual roles: 1. They may be used as Western Blot controls for Ras isoform-specific immunodetection2. Upon loading with GDP or GTP-γ-S (also included in the kit), Ras-GDP or Ras-GTP-γ-S proteins can be used to "spike" cell extracts thus serving as internal assay controls of signal specificity.
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