===
===under info was translated buy computer===Description
Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with the exon 5 defective variant of human estrogen receptor cDNA, cloned in pZeoSV vector, resistant to zeocin;
Original line: MCF7;
Derived from: pleural effusion
Organism: Human
Source: breast
Culture Properties: monolayer
Morphology: continuous culture, grown as monolayer, morphology epithelial-like
Recommended Medium: EMEM + 10% h.i. FBS
Quality Control: Sterility: mycoplasma negative, HOECHST and PCR
Storage and Shipping: Frozen with Culture medium + 50% FBS + 10% DMSO; ship in dry ice; store in liquid nitrogen