===
===under info was translated buy computer===Description
Species: human male 65 year old;
Tissue: skin;
Tumor: melanoma;
Derived from: a lymph node metastasis
Organism: Human
Source: skin
Culture Properties: monolayer
Morphology: continuous culture, grown as monolayer, morphology epithelial
Recommended Medium: RPMI-1640 medium supplemented with 2 mM L-glutamine and 10% h.i. FBS.
Quality Control: Sterility: mycoplasma negative, HOECHST and PCR
Storage and Shipping: Frozen with Culture medium + 10% DMSO; ship in dry ice; store in liquid nitrogen