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| Histone deacetylases (HDACs) play a critical role in transcriptional repression of the gene expression in eukaryotic cells through catalyzing the hydrolytic removal of acetyl groups from histone lysine residues. HDACs are tightly involved in cell cycle regulation, cell proliferation and in the development of human cancer. HDAC inhibition displays significant effects on apoptosis, cell cycle arrest and differentiation in cancer cells. HDAC inhibitors are currently being developed as potential anticancer agents. Three distinct families of HDACs have been described, comprising a group of at least 20 proteins in humans. HDAC2 is a class I histone deacetylase containing 488 amino acid residues. HDAC2 has been shown to interact directly with transcription factors and has been shown to deacetylate histone proteins H3 and H4. The major assay for measuring the expression or amount of HDAC2 protein currently is Western blot. This method requires electrophoresis and transfer process, which makes the assay inconvenient, time consuming, and has low throughput. The HDAC2 Assay Kit addresses these problems by using a unique procedure to measure amount of HDAC2. |
